Cytokeratin 6 Antibody [LHK6]

• Catalog Number: orb1252740
• Category: Antibodies
• Description: Cytokeratin 6 Antibody [LHK6]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LHK6
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG2a, kappa
• Immunogen: A synthetic peptide of 11 amino acids (GSSTIKYTTTS) from the C-terminus of human Cytokeratin 6 was used as the immunogen for this antibody.
• Antibody Type: Primary Antibody
• Concentration: 0.2 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: KRT6A
• UniProt ID: P02538
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Keratin, type II cytoskeletal 6A, Cytokeratin-6A,
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin/paraffin tissues is enhanced by digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 10-15 min at RT.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human bladder carcinoma and Cytokeratin 6 antibody.

SDS-PAGE Analysis of Purified, BSA-Free Cytokeratin 6 Antibody (clone LHK6). Confirmation of Integrity and Purity of the Antibody.