KRT6A Antibody

SKU: orb1252740

Description

KRT6A Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Human, Mouse
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 µg/mLIHC (FFPE): 0.5-1.0 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin/paraffin tissues is enhanced by digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 10-15 min at RT.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone No.: LHK6
• Immunogen: A synthetic peptide of 11 amino acids (GSSTIKYTTTS) from the C-terminus of human Cytokeratin 6 was used as the immunogen for this antibody.
• Target: KRT6A
• Purification: Protein G purified monoclonal antibody
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Keratin, type II cytoskeletal 6A, Cytokeratin-6A, CK-6A, Cytokeratin-6D, CK-6D, Keratin-6A, K6A, Type-II keratin Kb6, Hom s 5, KRT6A, K6A, KRT6D

IHC testing of FFPE human bladder carcinoma and Cytokeratin 6 antibody.

SDS-PAGE Analysis of Purified, BSA-Free Cytokeratin 6 Antibody (clone LHK6). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

KRT6A

UniProt

P02538