Cytokeratin 5/8 Antibody [SPM268]

• Catalog Number: orb1252413
• Category: Antibodies
• Description: Cytokeratin 5/8 Antibody [SPM268]
• Target: KRT5, KRT8
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Ferret, Hamster, Human, Mouse, Porcine, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein G affinity chromatography
• Immunogen: A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5/8 antibody.
• UniProt ID: P05787, P13647
• Tested applications: FC, IF, IHC-P, WB
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Cytokeratin 5/8 antibody should be determined by the researcher.1. For staining of formalin-fixed tissues, digest sections with Trypsin at 1mg/ml PBS, 15 min at RT2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: SPM268
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5/8 antibody (SPM268).