Cytokeratin 5/6 (Basal, Myoepithelial & Mesothelial Cell Marker) Antibody

• Catalog Number: orb750970
• Category: Antibodies
• Description: Mouse monoclonal antibody to KRT5 & KRT6
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: KRT5.6/2090
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Recombinant full-length human KRT5 protein
• Concentration: 200 μg/ml
• Dilution range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 58kDa (KRT5); 56kDa (KRT6A)
• Target: KRT5
• Entrez: 3852, 3853
• UniProt ID: P13647, P02538
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: Anti-CK5 antibody, anti-Keratin-5 antibody, anti-K
• Note: For research use only
• Application notes: Positive Control: HeLa, HepG2 or PC12 cells. Human prostate, tonsil or skin.
• Expiration Date: 12 months from date of receipt.