Cytokeratin 20 Antibody / CK20

• Catalog Number: orb2644099
• Category: Antibodies
• Description: This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: A recombinant human partial protein (amino acids 196-323) was used as the immunogen for this Cytokeratin 20 antibody.
• UniProt ID: P35900
• Tested applications: IHC-P, WB
• Dilution range: Immunohistochemistry (FFPE): 0.1-0.2ug/ml,Western blot: 1-2ug/ml
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: KRT20/3145
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Cytokeratin 20 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human colon carcinoma with Cytokeratin 20 antibody (clone KRT20/3145). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma with Cytokeratin 20 antibody (clone KRT20/3145). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human HT29 cell lysate with Cytokeratin 20 antibody (clone KRT20/3145). Predicted molecular weight ~46 kDa.

SDS-PAGE analysis of purified, BSA-free Cytokeratin 20 antibody (clone KRT20/3145) as confirmation of integrity and purity.