Cytokeratin 19 antibody (FITC)

• Catalog Number: orb154471
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to FITC which recognizes Cytokeratin 19 that belongs to the intermediate filament family. It expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. It involved in the organization of myofibers. Cytokeratin 19 is a specific marker of moderate to severe dysplasia and carcinoma in situ in oral cavity squamous epithelium, and measurement of Cytokeratin 19 may be a useful marker in diagnosing hepatoma.
• Clonality: Monoclonal
• Clone Number: A53-B/A2
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: MCF-7 human breast adenocarcinoma cell line
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: Cytokeratin 19
• Entrez: 3880
• UniProt ID: P08727
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-K19 antibody, anti-CK19 antibody, anti-CYK19
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Separation of MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) FITC antibody (concentration in sample 3 µg/ml, red-filled) from MCF-7 cells stained using mouse IgG2b isotype control (MPC-11) FITC antibody (concentration in sample 3 µg/ml, same as anti-Cytokeratin 19 FITC antibody concentration, black-dashed) in flow cytometry analysis (surface staining).