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Product Overview
Product Name Cytokeratin 18 antibody
Catalog Number orb248450
Species/HostMouse
ReactivityBovine, Canine, Hamster, Human, Porcine, Rat, Sheep
ConjugationUnconjugated
Tested applicationsFACS, ICC, IF, IHC-P, WB
Immunogen PMC-42 human breast carcinoma cells were used as the immunogen for this Cytokeratin 18 antibody.
Target Cytokeratin 18
Alternative Names
Product Properties
Form/Appearance 1X PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration 0.2 mg/ml
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Isotype IgG1
ClonalityMonoclonal
Purity Protein G Affinity Chromatography
Clone ID DC10
Entrez 3875
Product Description

Mouse monoclonal antibody to Cytokeratin 18

Application Notes
Dilution Range FACS: 0.5-1 μg, IF/ICC: 1-2 μg/ml, IP: 1-2 μg/500 μg, WB: 0.5-1 μg/ml, IHC-P: 0.5-1 μg/ml
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Validation Images
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human skin sweat gland tissue using Cytokeratin 18 antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human skin sweat gland tissue using Cytokeratin 18 antibody
Western blot analysis of human samples using Cytokeratin 18 antibody
Western blot analysis of human samples using Cytokeratin 18 antibody
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