Cytokeratin 18 antibody (Biotin)

• Catalog Number: orb43747
• Category: Antibodies
• Description: Mouse monoclonal antibody against Cytokeratin 18 conjugated to Biotin
• Clonality: Monoclonal
• Clone Number: DA-7
• Tested applications: ELISA, ICC, IHC-P, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Human breast carcinoma cell line PMC-42.
• Concentration: 1 mg/ml
• Dilution range: Western blotting: Recommended dilution: 1-2 μg/ml.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: Cytokeratin 18
• Entrez: 3875
• UniProt ID: P05783
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti-CYK 18 antibody, anti-CYK18 antibody, anti-CK
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 1-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody DA-7 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of biotinylated mouse anti-cytokeratin 18 monoclonal antibody followed by IRDye800-conjugated streptavidin. Cytokeratin 18 was detected at approximately 46 kDa.