Cytokeratin 15 Antibody [SPM190]

• Catalog Number: orb1252168
• Category: Antibodies
• Description: Cytokeratin 15 Antibody [SPM190]
• Target: KRT15
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Bovine, Human, Rat
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein G affinity chromatography
• Immunogen: An amino acid sequence from the C-terminal portion of the human protein was used as the immunogen for the Cytokeratin 15 antibody.
• UniProt ID: P19012
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlWB: 0.5-1 ug/mlImmunohistochemistry (FFPE & Frozen): 0.5-1 ug/ml for 30 min at RTOptimal dilution of the Cytokeratin 15 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: SPM190
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: KRT15, CK15, K15, K1CO
• Research Area: Epigenetics & Chromatin
• Note: For research use only

IHC testing of FFPE human skin with Cytokeratin 10 antibody (clone SPM190). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.