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Description
Research Area
Signal Transduction
Images & Validation
−| Tested Applications | ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | B3E4 |
| Immunogen | A synthesized peptide derived from human Cytokeratin 1 (600-644aa) |
| Target | KRT1 |
| Source/Expression System | 293F |
| Molecular Weight | Calculated M.W.: 70 kDa. Observed M.W.: 65 kDa. |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−AEI2; CK1; EHK; EHK1; EPPK; K1; KRT1A; NEPPK; Krt-2.1; Krt2-1; Krt86; Kb1; K2C1_HUMAN; KRT1; 67 kDa cytokeratin; Cytokeratin-1 (CK-1); Hair alpha protein; Keratin-1 (K1); Type-II keratin Kb1; KRTA; K2C1_MOUSE; K2C1_RAT; keratin 1; epidermolytic hyperkeratosis 1; keratin 1, type II
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Quick Database Links
Gene Symbol
KRT1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry















