Cytochrome P450 2E1/CYP2E1 Antibody

• Catalog Number: orb215944
• Category: Antibodies
• Description: Cytochrome P450 2E1/CYP2E1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Human
• Reactivity: Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CYP2E1 recombinant protein (Position: M1-Y310). Human CYP2E1 shares 73% and 74% amino acid (aa) sequences identity with mouse and rat CYP2E1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By HeatWestern blot, 0.1-0.5μg/ml, Mouse, Rat, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 56849 MW
• UniProt ID: P05181
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cytochrome P450 2E1;1.14.13.-;4-nitrophenol 2-hydr
• Note: For research use only
• Application notes: WB: The detection limit for CYP2E1 is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of CYP2E1 using anti-CYP2E1 antibody.Lane 1:rat liver Tissue;2:mouse liver Tissue.

IHC analysis of CYP2E1 using anti-CYP2E1 antibody. CYP2E1 was detected in paraffin-embedded section of mouse liver tissue.

IHC analysis of CYP2E1 using anti-CYP2E1 antibody. CYP2E1 was detected in paraffin-embedded section of rat liver tissue.