Cytochrome p450 2C19/CYP2C19 Antibody (monoclonal, 5G4)

• Catalog Number: orb654399
• Category: Antibodies
• Description: Cytochrome p450 2C19/CYP2C19 Antibody (monoclonal, 5G4)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5G4
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG2b
• Immunogen: A synthetic peptide corresponding to a sequence of human Cytochrome p450 2C19/CYP2C19 (QNQQSEFTIENLVITAADLLGAGTETT).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Mouse, RatImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 55 kDa
• UniProt ID: P33261
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Cytochrome p450 2C19/CYP2C19 using anti-Cytochrome p450 2C19/CYP2C19 antibody (orb654399). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cytochrome p450 2C19/CYP2C19 antigen affinity purified monoclonal antibody (Catalog # orb654399) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90502) with Tanon 5200 system. A specific band was detected for Cytochrome p450 2C19/CYP2C19 at approximately 55KD. The expected band size for Cytochrome p450 2C19/CYP2C19 is at 55KD.

IHC analysis of Cytochrome p450 2C19/CYP2C19 using anti-Cytochrome p450 2C19/CYP2C19 antibody (orb654399). Cytochrome p450 2C19/CYP2C19 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Cytochrome p450 2C19/CYP2C19 Antibody (orb654399) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90443) with DAB as the chromogen.