Cytochrome P450 1A2/CYP1A2 Antibody

• Catalog Number: orb308840
• Category: Antibodies
• Description: Cytochrome P450 1A2/CYP1A2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CYP1A2 recombinant protein (Position: H185-D320). Human CYP1A2 shares 69.9% and 72.1% amino acid (aa) sequence identity with mouse and rat CYP1A2, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 58294 MW
• UniProt ID: P05177
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cytochrome P450 1A2;1.14.14.1;CYPIA2;Cytochrome P
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of CYP1A2 using anti-CYP1A2 antibody.Lane 1:human HCCP tissue; 2:rat liver tissue; 3:mouse liver tissue.

IHC analysis of CYP1A2 using anti-CYP1A2 antibody. CYP1A2 was detected in a paraffin-embedded section of rat liver tissue.

IHC analysis of CYP1A2 using anti-CYP1A2 antibody. CYP1A2 was detected in a paraffin-embedded section of human liver cancer tissue.

IHC analysis of CYP1A2 using anti-CYP1A2 antibody. CYP1A2 was detected in a paraffin-embedded section of mouse liver tissue.