Cytochrome P450 1A1/CYP1A1 Antibody

• Catalog Number: orb308839
• Category: Antibodies
• Description: Cytochrome P450 1A1/CYP1A1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CYP1A1 recombinant protein (Position: H183-D320). Human CYP1A1 shares 81.2% amino acid (aa) sequence identity with both mouse and rat CYP1A1.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Mouse, - Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 58165 MW
• UniProt ID: P04798
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cytochrome P450 1A1;1.14.14.1;CYPIA1;Cytochrome P4
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of CACO-2 cells using anti-CYP1A1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of HeLa cells using anti-CYP1A1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of K562 cells using anti-CYP1A1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CYP1A1 using anti-CYP1A1 antibody.Lane 1:Rat Lung Tissue;2:Mouse Lung Tissue;3:Human Placenta Tissue;4:JURKAT Cell.

IF analysis of CYP1A1 using anti-CYP1A1 antibody. CYP1A1 was detected in immunocytochemical section of Caco-2 cells.

IHC analysis of CYP1A1 using anti-CYP1A1 antibody. CYP1A1 was detected in paraffin-embedded section of Mouse Kidney Tissue.

IHC analysis of CYP1A1 using anti-CYP1A1 antibody. CYP1A1 was detected in paraffin-embedded section of Rat Kidney Tissue.

IHC analysis of CYP1A1 using anti-CYP1A1 antibody. CYP1A1 was detected in paraffin-embedded section of Human Mammary Cancer Tissue.

IHC analysis of CYP1A1 using anti-CYP1A1 antibody.CYP1A1 was detected in frozen section of Mouse Kidney Tissue.

IHC analysis of CYP1A1 using anti-CYP1A1 antibody.CYP1A1 was detected in frozen section of Human Placenta Tissue.