Cystatin C/CST3 Antibody (monoclonal, 4H8)

• Catalog Number: orb527056
• Category: Antibodies
• Description: Cystatin C/CST3 Antibody (monoclonal, 4H8)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4H8
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: E. coli-derived human Cystatin C recombinant protein (Position: K31-A146).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells ELISA (Cap), 1-5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 15 kDa
• UniProt ID: P01034
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Nuclear factor 1 A-type; NF1-A; Nuclear factor 1/A
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-Cystatin C/CST3 antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Cystatin C/CST3 using anti-Cystatin C/CST3 antibody.Lane 1:human PC-3 cell;2:human CACO-2 cell;3:human placenta tissue;4:human A549 cell;5:human COLO320 cell;6:human HEK293 cell.

IF analysis of CST3 using anti-CST3 antibody. CST3 was detected in immunocytochemical section of MCF7 cells.

Sandwich ELISA - Recombinant human Cystatin C/CST3 protein standard curve.Use in combination with reagents from Human Cystatin C/CST3 ELISA Kit EZ-Set (DIY Antibody Pairs).

IHC analysis of Cystatin C/CST3 using anti-Cystatin C/CST3 antibody. Cystatin C/CST3 was detected in paraffin-embedded section of human colon cancer tissue.

IHC analysis of Cystatin C/CST3 using anti-Cystatin C/CST3 antibody. Cystatin C/CST3 was detected in paraffin-embedded section of human colon cancer tissue.

IHC analysis of Cystatin C/CST3 using anti-Cystatin C/CST3 antibody. Cystatin C/CST3 was detected in paraffin-embedded section of human glioma tissue.