Cystatin 8 antibody

• Catalog Number: orb20439
• Category: Antibodies
• Description: Goat polyclonal to Cystatin 8
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Dilution range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml
• Conjugation: Unconjugated
• MW: 16.3
• Target: Cystatin 8 (aa 23-34)
• Entrez: 10047
• Protein Sequence: DPKKNETGVLRK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CST8 antibody, anti cystatin 8 (cystatin-rela
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: In transfected HEK293 transiently expressing Human CST8 (myc and DYKDDDDK tagged), a band of approx. 23kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc tag antibody. Recommended concentration, 0.1-0.3 μg/ml.Experiment Notes: Purchase the +ve control! The transfected lysate used in QC is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.
• Expiration Date: 12 months from date of receipt.

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CST8 (RC210130) with C-terminal MYC tag probed with orb20439 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK294 probed with orb20601 (1mg/ml) in Lane