Cystathionine beta-synthase CBS Antibody

• Catalog Number: orb234268
• Category: Antibodies
• Description: Cystathionine beta-synthase CBS Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CBS recombinant protein (Position: A331-K551). Human CBS shares 83% amino acid (aa) sequence identity with both mouse and rat CBS.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 60587 MW
• UniProt ID: P35520
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cystathionine beta-synthase;4.2.1.22;Beta-thionase
• Note: For research use only
• Application notes: WB: The detection limit for CBS is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of CBS using anti-CBS antibody.Lane 1:human HeLa cell;2:human 293T cell;3:human K562 cell;4:rat liver tissue;5:rat pancrease tissue;6:mouse liver tissue;7:mouse pancrease tissue.

IHC analysis of CBS using anti-CBS antibody. CBS was detected in a paraffin-embedded section of human liver cancer tissue.