CYR61 antibody

• Catalog Number: orb539103
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CYR61
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 120-220 of human CYR61 (NP_001545.2).
• Dilution range: WB: 1:1000-1:2000
• Conjugation: Unconjugated
• MW: 41kDa
• UniProt ID: O00622
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Alternative names: CYR61, CCN1, GIG1, IGFBP10
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of A431 cells using CYR61 Rabbit pAb (orb539103) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CYR61 antibody (orb539103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.