CYP7A1 Antibody

• Catalog Number: orb1262824
• Category: Antibodies
• Description: CYP7A1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Rabbit
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This CYP7A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-504 amino acids from the C-terminal region of human CYP7A1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 58 kDa
• Target: CYP7A1
• UniProt ID: P22680
• NCBI: P22680
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Cholesterol 7-alpha-monooxygenase, CYPVII, Cholest
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of CYP7A1 Antibody in K562 (lane 1), HepG2 (lane 2) cell line lysates (35 ug/lane)