CYP2E1 Antibody

• Catalog Number: orb1249043
• Category: Antibodies
• Description: CYP2E1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-RKVIKNVAEVK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human Liver lysates (calculated MW of 56.8kDa according to NP_000764.1). Recommended concentration: 0.05-0.2ug/ml.Immunohistochemistry:In paraffin embedded Human Liver shows ER-like staining in hepatocytes. Recommended concentration, 3-5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CYP2E1
• UniProt ID: P05181
• NCBI: NP_000764.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CYP2E1, cytochrome P450, family 2, subfamily E, po
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human Liver lysates (calculated MW of 56.8kDa according to NP_000764.1). Recommended concentration: 0.05-0.2ug/ml.Immunohistochemistry:In paraffin embedded Human Liver shows ER-like staining in hepatocytes. Recommended concentration, 3-5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249043 (0.05 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249043 (3.8 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.