CYP1B1 Antibody

• Catalog Number: orb308841
• Category: Antibodies
• Description: CYP1B1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CYP1B1 recombinant protein (Position: R255-L480). Human CYP1B1 shares 85.4% and 84.5% amino acid (aa) sequence identity with mouse and rat CYP1B1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 60846 MW
• UniProt ID: Q16678
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cytochrome P450 1B1;1.14.14.1;CYPIB1;CYP1B1;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-CYP1B1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CYP1B1 using anti-CYP1B1 antibody.Lane 1:human MCF-7 cell; 2:human PC-3 cell; 3:rat heart tissue; 4:mouse heart tissue.

IHC analysis of CYP1B1 using anti-CYP1B1 antibody.CYP1B1 was detected in paraffin-embedded section of Mouse Kidney Tissue.

IHC analysis of CYP1B1 using anti-CYP1B1 antibody.CYP1B1 was detected in paraffin-embedded section of Rat Kidney Tissue.

IHC analysis of CYP1B1 using anti-CYP1B1 antibody.CYP1B1 was detected in paraffin-embedded section of Human Liver Cancer Tissue.