CYP17A1 Antibody

• Catalog Number: orb1247634
• Category: Antibodies
• Description: CYP17A1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Feline
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-LFKDGDQKLEK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in Human Ovary lysates (calculated MW of 57.4kDa according to NP_000093.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Adenal Gland shows strong granular staining of the cytoplasm in medulla cells. Recommended concentration: 3-5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CYP17A1
• UniProt ID: P05093
• NCBI: NP_000093.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CYP17A1, cytochrome P450, family 17, subfamily A,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in Human Ovary lysates (calculated MW of 57.4kDa according to NP_000093.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Adenal Gland shows strong granular staining of the cytoplasm in medulla cells. Recommended concentration: 3-5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247634 (0.3 ug/ml) staining of Human Ovary lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247634 (3.8 ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.