Cynomolgus M-CSF R / CSF1R / CD115 Protein

• Catalog Number: orb18114
• Category: Proteins
• Description: Colony stimulating factor 1 receptor (CSF1R) is also known as macrophage colony-stimulating factor receptor (M-CSFR), CD115 Cluster of Differentiation 115 (CD115), C-FMS, CSFR, FIM2, FMS, and is a member of the typeⅢ subfamily of receptor tyrosine kinases (RTKs). CSF1R is a receptor for a cytokine called colony stimulating factor 1, The protein encoded by the CSFR1 gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most, if not all, of the biological effects of this cytokine. Ligand binding activates CSFR1 through a process of oligomerization and transphosphorylation . Mutations in CSF1R are associated with chronic myelomonocytic leukemia and type M4 acute myeloblastic leukemia. Increased levels of CSF1R1 are found in microglia in Alzheimer's disease and after brain injuries. The increased receptor expression causes microglia to become more active. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.
• Reactivity: Monkey
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 81.9 kDa
• Target: M-CSF R
• Source: Cynomolgus M-CSF R, Fc Tag (orb18114) is expressed from human 293 cells (HEK293). It contains AA Ile 99 - Pro 596 (Accession # G7P8P1).
• Expression System: HEK293
• Biological Activity: Immobilized Biotinylated Human M-CSF, His, at 2 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Cynomolgus M-CSF R, Fc Tag with a linear range of 1-39 ng/mL (QC tested).
• Expression Region: Ile 99 - Pro 596
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: Tris with Glycine, Arginine and NaCl, pH7.5
• Alternative names: CSF1R,C-FMS,CD115,CSFR,FIM2,FMS,M-CSFR
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 81.9 kDa. As a result of glycosylation, the protein migrates as 110-125 kDa under reducing (R) condition, and 200- 240 kDa under non-reducing (NR) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

References

Buile, Dace et al. Evaluation of the Multiple Tissue Factors in the Cartilage of Primary and Secondary Rhinoplasty in Cleft Lip and Palate Patients Pediatr Rep, 14, 419-433 (2022)

PubMed: 36278554

SDS-PAGE analysis of Primate M-CSF R protein

Immobilized Biotinylated Human M-CSF, His, Avitag at 2 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Cynomolgus M-CSF R, Fc Tag (Cat. No. orb18114) with a linear range of 1-39 ng/mL (QC tested).