Cyld antibody

• Catalog Number: orb19453
• Category: Antibodies
• Description: Goat polyclonal antibody to Cyld
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Mouse, Rat
• Dilution range: ELISA: 1:32000, WB: 0.5-1.5 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 107
• Target: Cyld (mouse)
• Protein Sequence: DSQQSKSKNPWYIDE
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CYLD antibody, anti cylindromatosis (turban t
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 125kDa band observed in mouse and rat brain lysates (calculated MW of 107kDa according to mouse NP_775545.1). Recommended concentration: 0.5-1.5 μg/ml.
• Expiration Date: 12 months from date of receipt.

References

Yang, Xifeng et al. Downregulation of miR‑181b inhibits human colon cancer cell proliferation by targeting CYLD and inhibiting the NF‑κB signaling pathway Int J Mol Med, 46, 1755-1764 (2020)

PubMed: 32901872

orb19453 (0.5ug/ml) staining of rat brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Mouse Brain using Cyld antibody