Cyclophilin B/PPIB Antibody

• Catalog Number: orb402523
• Category: Antibodies
• Description: Cyclophilin B/PPIB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human Cyclophilin B recombinant protein (Position: K158-E216).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 19 kDa
• UniProt ID: P23284
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Peptidyl-prolyl cis-trans isomerase B; PPIase B; 5
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U937 cells using anti-Cyclophilin B antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Cyclophilin B using anti-Cyclophilin B antibody.Lane 1:human placenta tissue;2:human U87 cell;3:human HeLa cell;4:mouse HEPA1-6 cell;5:rat PC-12 cell;6:rat RH35 cell;7:mouse NIH/3T3 cell;8:mouse SP2/0 cell.

IF analysis of Cyclophilin B using anti-Cyclophilin B antibody. Cyclophilin B was detected in immunocytochemical section of U20S cells.

IHC analysis of Cyclophilin B using anti-Cyclophilin B antibody.Cyclophilin B was detected in paraffin-embedded section of human placenta tissue.

IHC analysis of Cyclophilin B using anti-Cyclophilin B antibody.Cyclophilin B was detected in paraffin-embedded section of human lung cancer tissue.

IHC analysis of Cyclophilin B using anti-Cyclophilin B antibody.Cyclophilin B was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of Cyclophilin B using anti-Cyclophilin B antibody.Cyclophilin B was detected in paraffin-embedded section of human rectal cancer tissue.

IHC analysis of Cyclophilin B using anti-Cyclophilin B antibody.Cyclophilin B was detected in paraffin-embedded section of rat thyroid gland tissue.