Cyclophilin A/PPIA Antibody

• Catalog Number: orb19174
• Category: Antibodies
• Description: Cyclophilin A/PPIA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Cyclophilin A recombinant protein (Position: T116-E165). Human Cyclophilin A shares 98% and 95.9% amino acid (aa) sequence identity with mouse and rat Cyclophilin A, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Western blot, 0.1-0.5μg/ml, Human, Mouse, RatImmunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 18012 MW
• UniProt ID: P62937
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Peptidyl-prolyl cis-trans isomerase A;PPIase A;5.2
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of THP-1 cells using anti-PPIA antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of K562 cells using anti-PPIA antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U937 cells using anti-PPIA antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Cyclophilin A using anti-Cyclophilin A antibody.Lane 1:human THP-1 cell; 2:human HepG2 cell; 3:human MOLT-4 cell; 4:human Daudi cell; 5:rat lung tissue; 6:rat brain tissue; 7:mouse lung tissue; 8:mouse brain tissue.

IF analysis of Cyclophilin A using anti-Cyclophilin A antibody.Cyclophilin A was detected in immunocytochemical section of A549 cell.

IHC analysis of Cyclophilin A using anti-Cyclophilin A antibody. Cyclophilin A was detected in paraffin-embedded section of mouse intestine tissues.

IHC analysis of Cyclophilin A using anti-Cyclophilin A antibody. Cyclophilin A was detected in paraffin-embedded section of rat spleen tissues.

IHC analysis of Cyclophilin A using anti-Cyclophilin A antibody. Cyclophilin A was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of Cyclophilin A using anti-Cyclophilin A antibody.Cyclophilin A was detected in immunocytochemical section of THP-1 cell.

IHC analysis of Cyclophilin A using anti-Cyclophilin A antibody.Cyclophilin A was detected in immunocytochemical section of THP-1 cell.

IHC analysis of Cyclophilin A using anti-Cyclophilin A antibody.Cyclophilin A was detected in immunocytochemical section of THP-1 cell.

IHC analysis of Cyclophilin A using anti-Cyclophilin A antibody.Cyclophilin A was detected in immunocytochemical section of THP-1 cell.