Cyclin K Antibody

• Catalog Number: orb1527522
• Category: Antibodies
• Description: Cyclin K Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 530 and 580
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Cyclin K
• UniProt ID: O75909
• NCBI: NP_001092872.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: CPR4; cyclin-K
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Cyclin K by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Jurkat, HeLa, MCF-7, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin K antibody (orb1527522) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse Cyclin K by western blot. Samples: Whole cell lysate (50 µg) from CT26, TCMK-1, BW5147.3, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin K antibody (orb1527522) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Cyclin K by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin K antibody (orb1527522) used for IP at 6 µg per reaction. Cyclin K was also immunoprecipitated by a previous lot of this antibody (orb1527522) and a second antibody against a different epitope of Cyclin K.

Detection of human Cyclin K by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Cyclin K orb1527522 used at a dilution of 1: 5, 000 (0.2 µg/ml). Detection: DAB