Cyclin D1 antibody (PE)

• Catalog Number: orb387897
• Category: Antibodies
• Description: Mouse monoclonal antibody against Cyclin D1 conjugated to PE
• Clonality: Monoclonal
• Clone Number: DCS-6
• Tested applications: FC
• Reactivity: Human, Mouse, Primate, Rat
• Isotype: Mouse IgG2a
• Immunogen: recombinant human cyclin D1 (amino acids 1-295)
• Concentration: 0.1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1 μg/ml. Intracellular staining.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: Cyclin D1
• Entrez: 595
• UniProt ID: P24385
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CYC-D1 antibody, anti-CCND1 antibody, anti-Bc
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1 μg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Separation of HUVEC cells stained using anti-Cyclin D1 (DCS-6) PE antibody (concentration in sample 15 µg/ml, red-filled) from HUVEC cells stained using mouse IgG2a isotype control (MOPC-173) PE antibody (concentration in sample 15 µg/ml, black-dashed) in flow cytometry analysis (intracellular staining) of HUVEC cell suspension.

Flow cytometry intracellular staining pattern of HUVEC cells stained using anti-Cyclin D1 (DCS-6) PE antibody (concentration in sample 15 µg/ml).