Cyclin B1 Antibody (PE)

• Catalog Number: orb1088479
• Category: Antibodies
• Description: Cyclin B1 is a regulatory protein involved in mitosis. It complexes with p34(cdc2) to form maturation-promoting factor (MPF), and is necessary for proper control of the G2/M phase transition of the cell cycle. It is expressed in tissues containing proliferating cells, such as lymph node, testis et al.
• Clonality: Monoclonal
• Clone Number: V152
• Tested applications: FC
• Reactivity: Mammal
• Isotype: Mouse IgG1
• Immunogen: Recombinant His-tagged hamster cyclin B1
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: Cyclin B1
• Entrez: 891
• UniProt ID: P14635
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Separation of K562 cells stained using anti-human Cyclin B1 (V152) PE antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from K562 cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 6 μg/ml, same as Cyclin B1 PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of K562 cell suspension.