Cyclin A2/CCNA2 Antibody

• Catalog Number: orb259601
• Category: Antibodies
• Description: Cyclin A2/CCNA2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Cyclin A2 recombinant protein (Position: A10-K168). Human Cyclin A2 shares 74.5% amino acid (aa) sequence identity with mouse Cyclin A2.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 48551 MW
• UniProt ID: P20248
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cyclin-A2;Cyclin-A;CCNA2;CCN1, CCNA;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-Cyclin A2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of Cyclin A2 using anti-Cyclin A2 antibody.Lane 1:human K562 cell; 2:human HL-60 cell; 3:human MCF-7 cell; 4:rat stomach tissue; 5:rat testis tissue; 6:mouse stomach tissue.

IF analysis of Cyclin A2 using anti-Cyclin A2 antibody. Cyclin A2 was detected in an immunocytochemical section of A431 cells.

IHC analysis of Cyclin A2 using anti-Cyclin A2 antibody. Cyclin A2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue.