CYBB antibody

• Catalog Number: orb20254
• Category: Antibodies
• Description: Goat polyclonal to CYBB
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Canine, Human
• Dilution range: ELISA: 1:64000, IHC-P: 1-2µg/ml
• Conjugation: Unconjugated
• MW: 65.3
• Target: CYBB / GP91-PHOX
• Entrez: 1536
• Protein Sequence: SYLNFARKRIKNP
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CYBB antibody, anti cytochrome b-245, beta po
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 85Da band observed in Human Peripheral Blood Mononucleocytes ysates (calculated MW of 65.3kDa according to NP_000388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Teahan et al, Nature. 1987 Jun 25-Jul 1;327(6124):720-1. PMID: 3600769). Recommended concentration: 0.03-0.1 μg/ml.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining with CYBB antibody.

orb20254 (0.03ug/ml) staining of Human Peripheral Blood Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.