Cyasterone

SKU: orb1222617

Description

Cyasterone was demonstrated potential inhibition effect in skin carcinoma cells.

Key Properties

• CAS Number: 17086-76-9
• MW: 520.66
• Purity: >98% (HPLC)
• Formula: C₂₉H₄₄O₈
• SMILES: O1C(=O)[C@H]([C@@H]([C@H]1C)C[C@H]([C@@](C)([C@H]1CC[C@]2(C3=CC(=O)[C@@H]4C[C@H]([C@H](C[C@@]4([C@H]3CC[C@]12C)C)O)O)O)O)O)C
• Target: EGFR
• Solubility: In Vitro: DMSO : 100 mg/mL (192.07 mM)

Bioactivity

• In Vivo:
  Cyasterone (intraperitoneal injection; 5 mg/kg, 10 mg/kg and 15 mg/kg; 21 days) has anti-proliferation effect In vivo and inhibits MGC823 cells xenografted tumor growth In vivo with few changes in body weights. Animal model: MGC823 xenograft tumor in BALB/C-nu mice. Dosage: 5 mg/kg, 10 mg/kg and 15 mg/kg. Administration: Intraperitoneal injection. Result: Inhibited MGC823 xenograft tumor growth In vivo.
• In Vitro:
  Cyasterone exerts cytotoxicity on multiple cell lines: HeLa (IC50 = 77.24 μg/ml); HepG-2 (IC50 = 52.03 μg/ml); MCF-7 (IC50 = 82.07 μg/ml) andMCF-7 (IC50 = 82.07 μg/ml). And It has none cytotoxicity (IC50>400 μg/ml) on 3 types of carcinoma (HT-29, Caco-2, T47D) and 1 type of normal (NIH 3T3) cell lines. Cyasterone (0-60 μg/ml; 48 hours) causes a significantly decreasing of the cell proliferation. It inhibits cell growth as a dose-dependent manner, exhibits IC50 values of 38.50 μg/ml and 32.96 μg/ml, respectively. Cyasterone (0-60 μg/ml; 24 hours) decreases p-EGFR, p-MEK, and p-mTOR as a dose-dependent manner in A549 cells, it affects the MAPK signaling pathway activities. Cell Viability Assay Cell line: A549 cells and MGC823 cells. Concentration: 0 μg/ml, 20 μg/ml, 40 μg/ml, 60 μg/ml. Incubation time: 24 hours. Result: Inhibited cellproliferation as a dose-dependent manner. Western blot analysis. Cell line: A549 cells. Concentration: 0 μg/ml, 20 μg/ml, 40 μg/ml, 60 μg/ml. Incubation time: 24 hours. Result: Surpressed p-EGFR, p-MEK, and p-mTOR expression.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cyasterone | AI3-44890 | AI3 44890

Quick Database Links

Gene Symbol

EGFR