CXCR5 Recombinant Rabbit Monoclonal Antibody

SKU: orb704169

Description

CXCR5 Recombinant Rabbit Monoclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:1000-5000, Flow-Cyt=1ug/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 3B7
• Immunogen: A synthesized peptide derived from human CXCR5 (330-372aa)
• Target: CXCR5
• Molecular Weight: 45 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

BLR 1; BLR1; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1 GTP binding protein; C X C chemokine receptor type 5; C-X-C chemokine receptor type 5; CD 185; CD185; CD185 antigen; Chemokine C X C motif receptor 5; Chemokine CXC motif receptor 5; Chemokine receptor 5; CXC chemokine receptor type 5; CXC R5; CXC-R5; CXCR 5; CXCR-5; CXCR5_HUMAN; MDR 15; MDR-15; MDR15; MGC117347; Monocyte derived receptor 15; Monocyte-derived receptor 15.

Paraformaldehyde-fixed, paraffin embedded (rat spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CXCR5) Monoclonal Antibody, Unconjugated (orb704169) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Spleen (Mouse) Lysate at 40 ug, Lane 2: THP-1 (Human) Cell Lysate at 30 ug, Lane 3: U937 (Human) Cell Lysate at 30 ug, Lane 4: Molt-4 (Human) Cell Lysate at 30 ug, Lane 5: K562 (Human) Cell Lysate at 30 ug, Lane 6: A673 (Human) Cell Lysate at 30 ug, Primary: Anti-CXCR5 (orb704169) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 45 kD.

Quick Database Links

Gene Symbol

CXCR5

UniProt

P32302