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CXCR5 Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb704169
| Catalog Number | orb704169 |
|---|---|
| Category | Antibodies |
| Description | CXCR5 Recombinant Rabbit Monoclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Recombinant |
| Clone Number | 3B7 |
| Tested applications | FC, IF, IHC-Fr, IHC-P, WB |
| Predicted Reactivity | Mouse, Rat |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | Recombinant human CXCR5 |
| Antibody Type | Recombinant Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 45 kDa |
| Target | CXCR5 |
| UniProt ID | P32302 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | BLR 1; BLR1; Burkitt lymphoma receptor 1; Burkitt Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded (rat spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CXCR5) Monoclonal Antibody, Unconjugated (orb704169) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Spleen (Mouse) Lysate at 40 ug, Lane 2: THP-1 (Human) Cell Lysate at 30 ug, Lane 3: U937 (Human) Cell Lysate at 30 ug, Lane 4: Molt-4 (Human) Cell Lysate at 30 ug, Lane 5: K562 (Human) Cell Lysate at 30 ug, Lane 6: A673 (Human) Cell Lysate at 30 ug, Primary: Anti-CXCR5 (orb704169) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 45 kD.
