CXCL2 Antibody

• Catalog Number: orb1272519
• Category: Antibodies
• Description: CXCL2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGRO beta (human GRO-beta).
• Concentration: batch dependent
• Dilution range: ELISA:To detect hGRO beta by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGRO beta. Sandwich: To detect hGRO-β by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human GRO-β as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGRO-β.Western Blot:To detect hGRO beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: CXCL2
• UniProt ID: P19875
• NCBI: P19875
• Storage: GRO-B antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: GRO2, GROb, MIP2, MIP2A, SCYB2, MGSA-b, MIP-2a, CI
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

To detect Human GRO-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human GRO-beta (orb1272518) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human GRO-beta.

To detect human GRO-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect human GRO-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed paraffin-embedded sections of human colon/rectum adenocarcinoma tissue. The recommended concentration is 1.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.