CXCL10 Antibody

• Catalog Number: orb1272640
• Category: Antibodies
• Description: CXCL10 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3.3-1G1-2G7-2F6
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced in BALB/c mice using highly pure recombinant Human IP-10 as the immunizing antigen. This antibody was purified from cell culture by Protein A affinity chromatography.
• Concentration: batch dependent
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: CXCL10
• UniProt ID: P02778
• NCBI: P02778
• Storage: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: CXCL10, INP10, SCYB10, C-X-C motif chemokine 10, 1
• Note: For research use only
• Application notes: ELISA:Sandwich:In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant Human IP-10 when used with BIorbyt’s Biotinylated Antigen Affinity Purified anti-Human IP-10 (38-252) as the detection antibody at a concentration of approximately 0.25 μg/mL.Western Blot:To detect Human IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL (non-reducing) or at a concentration of 1.0-2.0 μg/mL (reducing). When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IP-10 is 0.20-0.40 ng/lane under non-reducing conditions and 2.00-4.00 ng/lane under reducing conditions.
• Expiration Date: 12 months from date of receipt.

To a sandwich ELISA (assuming 100 ul/well), a concentration of 1.0-2.0 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant Human IP-10 when used with Biotinylated Antigen Affinity Purified anti-Human IP-10 (orb1272429) as the detection antibody at a concentration of approximately 0.25 ug/ml.

To detect Human IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml (non-reducing) or at a concentration of 1.0-2.0 ug/ml (reducing). When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IP-10 is 0.20-0.40 ng/lane under non-reducing conditions and 2.00-4.00 ng/lane under reducing conditions.

To detect Human IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml (non-reducing) or at a concentration of 1.0-2.0 ug/ml (reducing). When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IP-10 is 0.20-0.40 ng/lane under non-reducing conditions and 2.00-4.00 ng/lane under reducing conditions.