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Catalog Number | orb1227445 |
---|---|
Category | Assays and Kits |
Description | CTLA4 Detection Set (Risk Free) |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | ELISA, FC, ICC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | Mouse monoclonal CTLA-4 antibodies were raised against the extracellular domain of human CTLA-4. |
Concentration | Antibody 1 mg/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Storage | Antibodies can be stored at 4˚°C for three months and -20˚°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | PBS containing 0.02% sodium azide. |
Note | For research use only |
Expiration Date | Please enquire. |
Western blot analysis of CTLA-4 in overexpressing 293 cells using orb1239307, orb1239311, and orb1239312 antibody at 1, 0.5, and 0.25 μg/ml, respectively.
Immunocytochemistry of CTLA-4 in overexpressing 293 cells using (A) orb1239307, (B) orb1239311, (C) orb1239312, and (D) control mouse IgG antibody at 5 μg/ml.
Immunofluorescence of CTLA-4 in overexpressing 293 cells using (A) orb1239307, (B) orb1239311, (C) orb1239312, and (D) control mouse IgG antibody at 20 μg/ml.
Immunofluorescence of CTLA-4 in human tonsil tissue using (A) orb1239307, (B) orb1239311, (C) orb1239312, and (D) control mouse IgG antibody at at 20 μg/ml.
Immunohistochemistry of CTLA-4 in human lymph node tissue using (A) orb1239307, (B) orb1239311, (C) orb1239312 and (D) control mouse IgG antibody at 5 μg/ml.
Immunohistochemistry of CTLA-4 in human tonsil tissue using (A) orb1239307, (B) orb1239311, (C) orb1239312 and (D) control mouse IgG antibody at 5 μg/ml.
Titration curve analysis of CTLA-4 mAbs to detect recombinant CTLA-4 in ELISA with orb1239307, orb1239311, and orb1239312 antibodies at decreasing concentrations.
A sandwich ELISA was performed using the anti-CTLA-4 mAbs orb1239307, orb1239311, orb1239312, as the capture antibodies for the CTLA-4 extracellular domain antigen with HRP-labeled Risk-Free anti-CTLA-4 mAbs as the detection antibodies.