CTLA4 antibody (PE)

• Catalog Number: orb222750
• Category: Antibodies
• Description: Mouse monoclonal antibody against CTLA4 conjugated to PE
• Clonality: Monoclonal
• Clone Number: BNI3
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Human CD152-IgG heavy chain fusion protein
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: CD152
• Entrez: 1493
• UniProt ID: P16410
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti-ALPS5 antibody, anti-CD 152 antibody, anti-CD
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human PHA stimulated lymphocytes stained using anti-human CD152 (BNI3) PE antibody (10 µl reagent / 100 µl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Separation of human CD152 positive CD3 positive lymphocytes (red-filled) from CD152 negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral whole blood stained using anti-human CD152 (BNI3) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human PHA stimulated peripheral whole blood stained using anti-human CD152 (BNI3) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).