CTBP2 Antibody

• Catalog Number: orb259603
• Category: Antibodies
• Description: CTBP2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Predicted Reactivity: Bovine, Canine, Hamster, Monkey, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CTBP2 recombinant protein (Position: H321-Q445). Human CTBP2 shares 99.2% and 98.4% amino acid (aa) sequence identity with mouse and rat CTBP2, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human, Mouse Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 48945 MW
• UniProt ID: P56545
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: C-terminal-binding protein 2;CtBP2;CTBP2;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-CTBP2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of A431 cells using anti-CTBP2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CTBP2 using anti-CTBP2 antibody.Lane 1:human A549 cell; 2:human PC-3 cell; 3:human THP-1 cell; 4:human HEK293 cell; 5:human CACO-2 cell; 6:human HELA cell; 7:rat C6 cell; 8:mouse NIH/3T3 cell.

IF analysis of CTBP2 using anti-CTBP2 antibody.CTBP2 was detected in paraffin-embedded section of mouse intestine tissues.

IF analysis of CTBP2 using anti-CTBP2 antibody.CTBP2 was detected in immunocytochemical section of U20S cell.

IF analysis of CTBP2 using anti-CTBP2 antibody.CTBP2 was detected in paraffin-embedded section of human colon cancer tissues.

IF analysis of CTBP2 using anti-CTBP2 antibody.CTBP2 was detected in immunocytochemical section of U20S cells.

IF analysis of CTBP2 using anti-CTBP2 antibody.P-cadherin was detected in paraffin-embedded section of human colon cancer tissues.

IF analysis of CTBP2 using anti-CTBP2 antibody.P-cadherin was detected in paraffin-embedded section of human colon cancer tissues.

IF analysis of CTBP2 using anti-CTBP2 antibody.P-cadherin was detected in paraffin-embedded section of mouse intestine tissues.

IHC analysis of CTBP2 using anti-CTBP2 antibody.CTBP2 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue.

IHC analysis of CTBP2 using anti-CTBP2 antibody.CTBP2 was detected in paraffin-embedded section of Rat Skeletal Muscle Tissue.

IHC analysis of CTBP2 using anti-CTBP2 antibody.CTBP2 was detected in paraffin-embedded section of Mouse Skeletal Muscle Tissue.