CSX1 / NKX2-5

• Catalog Number: orb20262
• Category: Antibodies
• Description: Goat polyclonal antibody to NKX2-5
• Target: CSX1 / NKX2-5
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Protein Sequence: PRAYSDPDPAKDPR
• RRID: AB_10749304
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-0.5 μg/ml, IHC-P: 10 μg/ml
• Application notes: WB: Approx. 38kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 34.9kDa according to Human NP_004378.1, 34.2kDa according to Mouse NP_032726.1 and 34.3kDa according to Rat NP_446103.2). Recommended concentration: 1-3µg/ml. Primary incu. IHC: Paraffin embedded Human Heart. Recommended concentration: 5-6µg/ml.
• Alternative names: NKX2-5; NK2 transcription factor related, locus 5
• Note: For research use only

1 µg/ml staining of Mouse Heart lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

5 µg/ml staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.