CSTA antibody

• Catalog Number: orb382603
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CSTA
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cystatin A. The exact sequence is proprietary.
• Dilution range: WB: 1:500:2000, IHC-P: 1:50:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CSTA
• Entrez: 1475
• UniProt ID: P01040
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-STF1 antibody, anti-STFA antibody, anti-Cysta
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Joost, Simon et al. Single-Cell Transcriptomics of Traced Epidermal and Hair Follicle Stem Cells Reveals Rapid Adaptations during Wound Healing Cell Rep, 25, 585-597.e7 (2018)

PubMed: 30332640

Western blot analysis of Cystatin A expression in HEK293T (A), Hela (B), NIH3T3 (C), mouse kidney (D) whole cell lysates. (Predicted band size: 11 kD; Observed band size: 11 kD)

Immunohistochemical analysis of Cystatin A staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.