CSRP2 antibody

• Catalog Number: orb19668
• Category: Antibodies
• Description: Goat polyclonal antibody to CSRP2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Equine, Human, Mouse, Rat
• Dilution range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 3-6 μg/ml
• Conjugation: Unconjugated
• MW: 21
• Target: CRP2 / CSRP2
• Entrez: 1466
• Protein Sequence: ERLGIKPESVQP
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CRP2 antibody, anti CSRP2 antibody, anti cyst
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 26kDa band observed in Human Heart and Kidney lysates and in Mouse and Rat Kidney lysates (calculated MW of 21.0kDa according to NP_001312.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Huber et al, J Biol Chem. 2000 Feb 25;275(8):5504-11; PMID: 10681529). In transfected HEK293 transiently expressing CRP2 a band of approx. 26kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19668 (0.1ug/ml) staining of mouse kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing CRP2 (RC201565) and probed with orb19668 (mock transfection in first lane), tested by Origene.

Western blot analysis of Human (A), Mouse (B) and Rat (C) Kidney lysates using CSRP2 antibody

Immunohistochemical staining of Human Lung using CSRP2 antibody