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Catalog Number | orb1247704 |
---|---|
Category | Antibodies |
Description | CSNK1D Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, WB |
Predicted Reactivity | Canine, Mouse, Rat |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-DREERLRHSRNPATR |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48kDa band observed in lysates of cell line HEK 293 (calculated MW of 47.3kDa according to NP_001884.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunofluorescence: Strong expression of the protein seen in the nuclei of U251 cells. Recommended concentration: 10ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | CSNK1D |
UniProt ID | P48730 |
NCBI | NP_620693.1, NP_001884.2 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | casein kinase 1, delta, HGNC:2452, CSNK1D, HCKID, Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48kDa band observed in lysates of cell line HEK 293 (calculated MW of 47.3kDa according to NP_001884.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunofluorescence: Strong expression of the protein seen in the nuclei of U251 cells. Recommended concentration: 10ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1247704 (0.3 ug/ml) staining of HEK 293 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1247704 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue).
ELISA, IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, WB | |
Human, Mouse, Porcine, Primate, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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