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Catalog Number | orb196272 |
---|---|
Category | Antibodies |
Description | Csk Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human CSK recombinant protein (Position: S2-G204). Human CSK shares 99% amino acid (aa) sequence identity with both mouse and rat CSK. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 50704 MW |
UniProt ID | P41240 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Tyrosine-protein kinase CSK;2.7.10.2;C-Src kinase; Read more... |
Note | For research use only |
Application notes | WB: The detection limit for CSK is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of CSK using anti-CSK antibody.Lane 1:recombinant human CSK protein 0.5 ng.
WB analysis of CSK using anti-CSK antibody.Lane 1:rat testis tissue; 2:rat thymus tissue; 3:mouse liver tissue; 4:human HeLa cell; 5:human Jurkat cell; 6:human A549 cell; 7:human MCF-7 cell; 8:mouse NIH/3T3 cell; 9:mouse NEURO cell.
IHC analysis of CSK using anti-CSK antibody.CSK was detected in paraffin-embedded section of human tonsil tissues.
IHC analysis of Csk using anti-Csk antibody.Csk was detected in paraffin-embedded section of human tonsil tissues.
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