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Catalog Number | orb2294095 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant CRYM. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 6B3 |
Tested applications | ELISA, IHC-P, IP, WB |
Reactivity | Human |
Isotype | IgG1 Kappa |
Immunogen | CRYM (NP_001879, 215 a.a. ~ 314 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | EWVKPGAHINAVGASRPDWRELDDELMKEAVLYVDSQEAALKESGDVLLSGAEIFAELGEVIKGVKPAHCEKTTVFKSLGMAVEDTVAAKLIYDSWSSGK |
NCBI | NP_001879 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
CRYM monoclonal antibody (M03), clone 6B3 Western Blot analysis of CRYM expression in Jurkat.
Detection limit for recombinant GST tagged CRYM is approximately 0.03 ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to CRYM on formalin-fixed paraffin-embedded human skeletal muscle. [antibody concentration 3 ug/ml].
Immunoprecipitation of CRYM transfected lysate using anti-CRYM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CRYM MaxPab rabbit polyclonal antibody.
Western Blot analysis of CRYM expression in transfected 293T cell line by CRYM monoclonal antibody (M03), clone 6B3. Lane 1: CRYM transfected lysate(33.8 KDa). Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (36.74 KDa).