CRX monoclonal antibody (M01), clone F6-C2

• Catalog Number: orb2294134
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant CRX.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: F6-C2
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: CRX (AAH16664, 1 a.a. ~ 300 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MMAYMNPGPHYSVNALALSGPSVDLMHQAVPYPSAPRKQRRERTTFTRSQLEELEALFAKTQYPDVYAREEVALKINLPESRVQVWFKNRRAKCRQQRQQQKQQQQPPGGQAKARPAKRKAGTSPRPSTDVCPDPLGISDSYSPPLPGPSGSPTTAVATVSIWSPASESPLPEAQRAGLVASGPSLTSAPYAMTYAPASAFCSSPSAYGSPSSYFSGLDPYLSPMVPQLGGPALSPLSGPSVGPSLAQSPTSLSGQSYGAYSPVDSLEFKDPTGTWKFTYNPMDPLDYKDQSAWKFQIL
• NCBI: AAH16664
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged CRX is approximately 1 ng/ml as a capture antibody.

Western Blot analysis of CRX expression in transfected 293T cell line by CRX monoclonal antibody (M01), clone F6-C2. Lane 1: CRX transfected lysate(32.261 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of CRX over-expressed 293 cell line, cotransfected with CRX Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CRX monoclonal antibody (M01), clone F6-C2 (Cat # orb2294134). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (42.5 KDa).