CRK (phospho-Y221) antibody

• Catalog Number: orb515487
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CRK (phospho-Y221)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y221 of human CRK protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IF/ICC: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CRK
• Entrez: 54245, 12928, 1398
• UniProt ID: Q64010, P46108, Q63768
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Adapter molecule crk; Proto-oncogene c-Crk; p38
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CRK (Phospho-Y221) expression in Hela (A), THP1 (B), mouse liver (C), rat liver (D) whole cell lysates. (Predicted band size: 33 kD; Observed band size: 46 kD)

Immunofluorescent analysis of CRK (Phospho-Y221) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.