CRHR1 antibody

• Catalog Number: orb19649
• Category: Antibodies
• Description: Goat polyclonal antibody to CRHR1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 47.7
• Target: CRHR1 / CRF-R (aa 107 - 117)
• Entrez: 1394
• Protein Sequence: NEEKKSKVHYH
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CRHR1 antibody, anti CRF-R antibody, anti cor
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: This product was successfully used on cryosection of mouse brain (PMID: 22570383)WB: Approx 60-70kDa band observed in human brain (cerebellum) and human colon lysates (calculated MW of 47.7kDa according to NP_004373.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu et al, Mol Pharmacol. 2001 Mar;59(3):485-92; PMID: 11179443). Recommended concentration: 0.1-0.3 μg/ml.Experiment Notes: Immunofluorescence: This product was successfully used in immunofluorescence on mouse brain at 2ug/ml when incubated at 4C for 40h.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of mouse cortex using CRHR1 antibody

Western blot analysis of Human Colon (Lane 1), Cerebellum (Lane 2), Rat Brain (Lane 3) and Mouse spinal Cord (Lane 4) lysates using CRHR1 antibody