CREB-1 Rabbit Polyclonal Antibody

• Catalog Number: orb499581
• Category: Antibodies
• Description: CREB-1 Rabbit Polyclonal Antibody
• Target: CREB1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human CREB-1 (51-120/341aa)
• UniProt ID: P16220
• MW: 37 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Active transcription factor CREB; cAMP response el
• Research Area: DNA and RNA Research, Epigenetics and Nuclear Sign
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): Hela cells (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C). Primary Antibody (green line): Rabbit Anti-phospho-CREB-1 antibody (orb499581), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Sample: A549 (Human) Lysate at 30 ug, Primary: Anti-CREB-1 (orb499581) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37 kD, Observed band size: 37 kD.

Sample: Lane 1: Cerebrum (Rat) Lysate at 40 ug, Lane 2: Kidney (Mouse) Lysate at 40 ug, Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 4: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-CREB-1 (orb499581) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 47 kD.

Sample: U251 (Human) Lysate at 30 ug, Primary: Anti-CREB-1 (orb499581) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37 kD, Observed band size: 37 kD.