cPLA2 (phospho-Ser505) antibody

• Catalog Number: orb769474
• Category: Antibodies
• Description: Rabbit polyclonal antibody to cPLA2 (phospho-Ser505)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human c-PLA2 around the phosphorylation site of Ser505. AA range:471-520
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 110
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-PLA2G4A antibody, anti-CPLA2 antibody, anti-P
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of VEC using p-cPLA2 (S505) antibody. Antibody was diluted at 1:1000.

Immunofluorescence analysis of Hela cell. 1, cPLA2 (phospho Ser505) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using c-PLA2 (Phospho-Ser505) Antibody.