CPI17 alpha/PPP1R14A Antibody

• Catalog Number: orb19175
• Category: Antibodies
• Description: CPI17 alpha/PPP1R14A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CPI17 alpha recombinant protein (Position: L30-Q126). Human CPI17 alpha shares 83.5% and 84.5% amino acid (aa) sequence identity with mouse and rat CPI17 alpha, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 16693 MW
• UniProt ID: Q96A00
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Protein phosphatase 1 regulatory subunit 14A;17 kD
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-CPI17 alpha antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CPI17 alpha using anti-CPI17 alpha antibody.Lane 1:rat brain tissue;2:mouse brain tissue;3:PANC-1 cell.

IF analysis of CPI17 alpha using anti-CPI17 alpha antibody. CPI17 alpha was detected in immunocytochemical section of U20S cells.

IHC analysis of CPI17 alpha using anti-CPI17 alpha antibody. CPI17 alpha was detected in paraffin-embedded section of mouse testis tissues.

IHC analysis of CPI17 alpha using anti-CPI17 alpha antibody. CPI17 alpha was detected in paraffin-embedded section of human placenta tissues.

IHC analysis of CPI17 alpha using anti-CPI17 alpha antibody. CPI17 alpha was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of CPI17 alpha using anti-CPI17 alpha antibody. CPI17 alpha was detected in paraffin-embedded section of human lung cancer tissues.

IHC analysis of CPI17 alpha using anti-CPI17 alpha antibody. CPI17 alpha was detected in paraffin-embedded section of rat lung tissues.