COX5A Antibody

• Catalog Number: orb1520821
• Category: Antibodies
• Description: COX5A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 100 and 150
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 20 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: COX5A
• UniProt ID: P20674
• NCBI: P20674.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: COX; COX-VA; cytochrome c oxidase polypeptide Va;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse COX5A by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-COX5A antibody (orb1520821) used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human COX5A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COX5A antibody (orb1520821) used for IP at 2 µg per reaction.